Figure 5. Selectin Binding and Selectin-Mediated Leukocyte Rolling.
(a) Flow cytometry analysis of HL-60 cells treated for 5 days with 200 μM each of (2) or (8) leads to decreases in anti-Sialyl Lewis X antibody as well as recombinant E- and P-selectin binding. This leads to higher rolling velocities (at 3 dynes/cm2) of these cells on selectin coated surfaces (b) and a decreased shear resistance on E- but not P-selectin coated surfaces (c). *** corresponds to p < 0.0001.