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Quantification of nucleotide sugars in cell extracts (2.2 × 108 cells) as determined by HPLC
| Treatment | Nucleotide Sugar | Amount (μg) |
|---|---|---|
| DMSO | GDP-Fuc (4) | 3.88 ± 0.05 |
| CMP-NeuAc (10) | 2.59 ± 0.05 | |
| 2F-Fuc (2) | GDP-Fuc (4) | Not Detected |
| 2F GDP-2F-Fuc (5) | 62.46 ± 0.49 | |
| CMP-NeuAc (10) | 2.20 ± 0.04 | |
| 3F-NeuAc (8) | GDP-Fuc (4) | 4.39 ± 0.02 |
| CMP-NeuAc (10) | Not Detected | |
| 3F CMP-NeuAc (11) | 65.18 ± 0.18 |
