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. Author manuscript; available in PMC: 2013 Oct 4.
Published in final edited form as: Oncogene. 2012 May 21;32(14):1794–1810. doi: 10.1038/onc.2012.200

Fig. 9.

Fig. 9

Effects of serum starvation and D1 on RSK4. MB231 (A) and MCF7 (B) cells sorted for a pMIG retrovirus that contains D1 or its K112E mutant were cultured in duplicate plates. One of the plates was then withdrawn from 10% serum for 48 hours. The cell lysates were immunoblotted with indicated antibodies and then were stripped and re-blotted with β-actin antibody to visualize the protein loading. Note that withdrawal of D1 or K112E expressing cells from serum increases the levels of most proteins detected by most antibodies, but the endogenous 90-kD protein is only detected in MCF7 cells by the ab42100 antibody (arrow).