Table 2.

Effects of serum starvation on the cell cycle distribution of AsPC-1 cells

Time	Clone	%G1	% S	%G2-M	%subG1
24 hr	Vector-0%	70.95±4.6	22.67±4.25	6.38±1.98	0.79±0.23
	Vector-10%	25.91±6.11a	68.56±4.85a	5.53±1.66	1.34±0.58
	hRSK4-0%	51.55±8.81c	30.67±6.56c	17.78±2.36c	1.44±0.4
	hRSK4-10%	23.25±5.79a,c	62.99±4.8a,c	13.76±1.87a,c	2.29±0.29
	ΔE21-0%	43.47±2.13c	37.36±3.53c	19.17±2.0c	2.22±0.48
	ΔE21-10%	24.72±4.98a	71.04±6.27a	4.24±1.72a,b	2.73±0.6
48 hr	Vector-0%	75.33±4.5	18.71±3.21	5.96±1.55	1.54±0.62
	Vector-10%	31.99±2.19a	61.53±3.81a	6.48±1.03	2±0.46
	hRSK4-0%	53.61±4.04c	22.91±2.94c	23.48±2.45c	2.46±0.74
	hRSK4-10%	23.43±1.66a,c	58.21±1.89a,c	18.36±1.94a,c	2.51±0.37
	ΔE21-0%	45.68±3.11c	29.71±2.48c	24.62±1.63c	2.98±0.94
	ΔE21-10%	31.23±2.07a	62.56±2.15a	6.21±1.66a,b	2.61±0.43
72 hr	Vector-0%	79.98±3.81	13.54±2.15	6.48±2.0	1.73±0.76
	Vector-10%	33.57±1.02a	60.22±3.34a	6.21±0.34	2.33±0.54
	hRSK4-0%	54.48±1.56c	28.6±5.11c	16.92±1.56c	3.28±0.48
	hRSK4-10%	21.53±1.5a,c	61.1±3.13a,c	17.38±1.45a,c	1.44±0.59
	ΔE21-0%	45.11±0.9c	35.78±4.78c	19.11±1.0c	4.7c±0.58
	ΔE21-10%	30.86±1.45a,b	61.47±1.66a	7.66±1.67a,b	2.14±0.48
96 hr	Vector-0%	76.56±2.34	16.25±2.38	7.18±1.448	4.53±0.58
	Vector-10%	29.4±5.36a	63.77±2.33a	6.83±1.34	2.5±0.66
	hRSK4-0%	54.29±1.99c	29.59±2.25c	16.13±1.48c	6.24±0.38c
	hRSK4-10%	14.47±3.19a,c	70.92±2.28a,c	14.61±2.43a,c	2.17±0.8a,c
	ΔE21-0%	47.64±3.12c	34.95±1.86c	17.41±2.34c	7.31±0.65c
	ΔE21-10%	29.44±1.01a,b	63.34±3.3a	7.22±1.41a,b	2.53±0.46a

Note: Data are presented as Mean ± SD. G1, S and G2 phase cells sum to 100% of viable cells. Sub-G1 are attached but dead cells.

^asignificantly different from 0% serum counterpart (p<0.05);

^bsignificantly different from the intact hRSK4 counterpart (p<0.05);

^csignificantly different from the vector counterpart (p<0.05).