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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Methods. 2012 Apr 6;57(2):179–186. doi: 10.1016/j.ymeth.2012.03.033

Figure 2.

Figure 2

Single-molecule manipulation and visualization of λ DNA. (A) The sequence of 12 bp single stranded ends of λ DNA. (B) A cartoon demonstration of the experimental setup used for stretching and imaging DNA. (C) SYTOX images of a singly-tethered λ DNA molecule in the absence (i) and presence (ii) of buffer flow. (iii) SYTOX image of a doubly-tethered λ DNA molecule stretched to 78% of the contour-length in the absence of flow (iv) A λ DNA molecule was stretched to 88% of the contour length by including 100 µM chloroquine in buffer (bar 2 µm). Adapted with permission from [30].