Fig. 3.

Correlation of ¹³C chemical shift differences, Δδ = Δω/(2πγB₀), between the folded and unfolded states of CspB derived from L-TROSY-CPMG relaxation dispersions under native conditions or measured directly from ¹H–¹³C HSQC spectra of the native and progressively urea-denatured states. The signs of the shift differences measured from spectra were also used for the CPMG-derived shift difference. Standard errors of the fitted parameters (vertical axis) were determined from the covariance matrix, whereas the uncertainties of the HSQC-derived shift differences are negligibly small. Sizeable uncertainties are observed only for F49δ*, which has a chemical shift difference close to zero, and H29ε1, which has low signal intensity (Fig. S1); for other residues the error bars are smaller than, or similar to, the size of the symbols