Table 4.
Structural parameters obtained from fitting of reflectivity profiles shown in figure 9, relating to the binding of the MTS and GMBS layer and the subsequent anti-hPSA antibody layer at the solid–buffer interface. (Symbols are the same as defined in table 3.)
| sample/concentration (mg l−1) | τ (Å) | (ρ ± 0.05) e−6 (Å−2) | φantibody/φBSA/φantigen (±2) (%) | Γantibody/ΓBSA/Γantigen (mg m−2) | Γtotal ± 0.1 (mg m−2) |
|---|---|---|---|---|---|
| SiO2 | 12 ± 2 | 3.41 | |||
| MTS and GMBS | 20 ± 2 | 3.95 | 51 | ||
| antibody/10 | 48 ± 2 | 5.7 | 22 | 1.5 ± 0.1 | 1.5 |
| antibody/10 + BSA/50 | 46 ± 2 | 5.7 | 22 | 1.44 ± 0.1 | 1.44 |
| antibody/10 + BSA/50 + antigen/5 | 46 ± 2 | 5.6 | 22/0/3 | 1.44 ± 0.1/0/0.19 ± 0.03 | 1.63 |