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. 2012 Sep;92(3):413–419. doi: 10.1189/jlb.0711344

Figure 6. Migration of primed T cells.

Figure 6.

(A) In vitro-primed CD4 cells were adoptively transferred into syngeneic WT C57BL/6 mice and visualized intravitally via multiphoton microscopy. Qtracker 655 (Life Technologies) was injected i.v. to highlight vasculature (red). CFSE-loaded T cells (green) were tracked (white line) for a 15-min time period at 16 h post-transfer. Original scale bar = 50 μm; n = 3. (B–D) T cell migration was quantified using a 3D volume and 2D slice from a total of three animals, and data were pooled. Each symbol represents an individual cell, and the horizontal line on each graph indicates the mean. (B) Average speed of T cells from a 2D image (open circles) was slower than as the same cells analyzed in 3D (12 μm/min and 10 μm/min, respectively; P=0.03). (C) No significant difference was seen in arrest coefficient in 3D (3 μm/min) versus 2D (10 μm/min). (D) No significant difference in meandering index was observed in 3D versus 2D analysis (0.48 vs. 0.44, respectively).