Figure 2. Peritoneal cavity and mesenteric microvasculature accumulation by neutrophils expressing or lacking ADAM17.

(A) Neutrophils from control and conditional ADAM17 knockout mice were labeled with distinct fluorophores (CFSE and CMTMR), mixed 1:1, and infused i.v. into C57BL/6J recipient mice, i.p.-injected with E. coli (5×10⁷ CFU), 2 h previously, as illustrated. (B) Peripheral blood and peritoneal lavage fluid were harvested 2 h later, and the proportion of total fluorescent cells in each sample, which represents neutrophils expressing (Control neut.) or lacking ADAM17 (ADAM17-null neut.), was determined by flow cytometry. The contour plots show representative data from four separate experiments. The indicated percentages represent the proportion of total cells in the top two quadrants. Results shown in the bar graph are expressed as the mean (±sd) of four mice in each group. *P < 0.01 versus control. (C) Neutrophils from control and conditional ADAM17 knockout mice were fluorescently labeled (CFSE), and equivalent cell numbers were i.v.-injected into separate C57BL/6J mice, 2 h after i.p. injection of LPS. Neutrophil interactions with the vascular endothelium of postcapillary venules were analyzed by fluorescent intravital microscopy of the exteriorized mesentery. Fluorescent neutrophils attached to the microvascular endothelium (rolling or firmly adhered) are expressed as the number of accumulated cells/250 μm/min. Results are expressed as the mean (±sd) of at least four mice in each group. **P < 0.001 versus control.