Figure 4. Selective inhibition of L-selectin shedding increases neutrophil influx into the infectious locus.

(A) Neutrophils from L(E) and control mice were examined in a competitive leukocyte migration assay, as described in Fig. 2A and B. Data are the mean (±sd) of four mice. *P < 0.05 versus control. (B) Conditional ADAM17 knockout, L(E), and control mice were injected i.p. with E. coli (5×10⁷ CFU). Four hours later, L-selectin surface expression levels on peripheral blood neutrophils were determined by flow cytometry [mean fluorescence intensity (MFI)], plasma levels of TNF-α were determined by ELISA, and neutrophil and bacteria levels in the peritoneal cavity were quantified. Results are expressed as the mean (±sd) of four to eight mice in each group. *P < 0.05 versus control; **P < 0.001 versus control.