Figure 5. Phenotype and functions of DCs.

(A) Representative FACS plots of a number of subpopulations of DCs in spleen. Flow cytometry strategy is shown in Supplemental Fig. 2A. Lymphoid CD4⁺ DCs are gated for CD11c⁺CD8⁻B220⁻, myeloid DCs as CD11c⁺B220⁻CD8⁻CD4⁻, plasmacytoid DCs B220⁺CD11b⁻, and lymphoid CD8⁺ DCs as CD11b⁻B220⁻CD4⁻CD8⁺. (B) Number of myeloid DCs in tumor tissue; cells are gated as CD45⁺CD4⁻CD8⁻B220⁻. Data correspond with the mean ± sem of three individual mice from three experiments. (C and D) MLR; DCs were isolated by FACS sorting from EL4-OVA tumor tissue and then mixed in different ratios with splenocytes from OT-I mice (C). MLR; splenocytes from tumor-bearing mice (EL4-OVA) were mixed in different ratios with splenocytes from OT-I mice (D). Data correspond with the mean ± sem of three individual mice from two experiments. *P < 0.05; **P < 0.01; data correspond with the mean ± sem.