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. Author manuscript; available in PMC: 2012 Aug 25.
Published in final edited form as: Methods Mol Biol. 2012;831:133–140. doi: 10.1007/978-1-61779-480-3_8

Figure 1.

Figure 1

(a) 1H-13C HMQC of [U-2H,12C], Ala-, Leu-, Met-, Val-, Ile-δ1-[13CH3] labeled protein. The methyl groups of all five amino acids can be labeled with no scrambling. (b) 1H-13C HMQC of the same protein as in (a, black) but prepared using 10% BIOEXPRESS (green). The Leu methyl groups are completely suppressed whereas the Val methyl groups are only minimally affected.