Figure 1.

(A) Urinary steroid metabolite profiles of WT vs 11β-HSD1 KO/DKO mice. Urine samples were collected from WT, 11β-HSD1 KO and DKO mice of both sexes using filter paper as in Materials and Methods section. Urinary steroids were subsequently extracted and analysed by GC/MS. Urinary steroid metabolites are shown as percentage 11-DHC metabolites as a proportion of total GC. **P<0·01 (n>4/group) between WT and KO of same sex; ^##P<0·01 (n>4/group) between sexes of same genotype; *P<0·05 (n>4/group) between WT and KO of same sex. (B) Urinary steroid metabolite profiles of WT vs H6PDH KO mice. Urine samples were collected from WT and H6PDH KO mice of both sexes on a daily basis using filter paper and were analysed as in (A). **P<0·01 (n>4/group).