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. Author manuscript; available in PMC: 2012 Nov 18.
Published in final edited form as: Angew Chem Int Ed Engl. 2011 Sep 28;50(47):11210–11214. doi: 10.1002/anie.201104649

Figure 1.

Figure 1

Characterization of semi-synthetic di-Ub using (a) HPLC and mass spectrometry analysis (for the K33 case) showing the correct molecular weight of 17195 Da (Calcd. 17194 Da) and (b) SDS-PAGE of the K33 and K48 chains.