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. Author manuscript; available in PMC: 2012 Aug 26.
Published in final edited form as: Cell Host Microbe. 2010 Mar 18;7(3):197–209. doi: 10.1016/j.chom.2010.02.009

Figure 1. VBF interacts with VIP1 and is upregulated by Agrobacterium infection.

Figure 1

Figure 1

(A) BiFC assay for the VBF-VIP1 interaction in microbombarded Arabidopsis leaves. (B) BiFC assay for the VBF-VIP1 interaction in agroinfiltrated N. benthamiana leaves. FBX is encoded by the At1g31350 gene. Free DsRed2 labels the cell cytoplasm and the nucleus and identifies the transformed cells. All images are projections of several confocal sections. (C) Yeast two-hybrid assay for the VBF-VIP1 interaction. The indicated dilutions of cell cultures were grown either in the absence (left panel) or in the presence of histidine (right panel). Lane 1, Gal4AD-VBF+LexA-VIP1; lane 2, Gal4AD-VBF+LexA-FBX; lane 3, Gal4AD+LexA-VIP1, lane 4, Gal4AD-VirE2+LexA-VIP1; lane 5, Gal4AD-VBF+LexA-lamin C; lane 6, Gal4AD-VBF+LexA-ASK1; lane 7, Gal4AD+LexA-ASK1. (D) RT-PCR analysis of the VBF gene expression following inoculation by Agrobacterium. Constitutively-expressed TUBULIN was used as internal control. (E) Q-PCR analysis of the VBF gene expression following inoculation by Agrobacterium. The data represent average values of three independent experiments with indicated standard deviations. (F) Expression of GUS reporter from the VBF promoter in Arabidopsis roots following inoculation by Agrobacterium. (-), (+) indicate mock-inoculated or Agrobacterium-inoculated plants, respectively.