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. Author manuscript; available in PMC: 2012 Aug 26.
Published in final edited form as: Cell Host Microbe. 2010 Mar 18;7(3):197–209. doi: 10.1016/j.chom.2010.02.009

Figure 3. Formation of ternary VIP1-VBF-ASK1 complexes in microbombarded N. benthamiana leaves.

Figure 3

(A) Multi-color bridge-BiFC assay. nCerulean/cCFP and nVenus/cCFP signals are indicated in blue and green, respectively; merged image represents overlay of both BiFC signals and DsRed2. Free DsRed2 labels the cytoplasm and the nucleus and identifies the transformed cells. (B) Coprecipitation. Lane 1, GFP-VIP1+His-VBF+HA-ASK1; lane 2, GFP-VIP1+HA-ASK1. Input, the GFP-VIP1+His-VBF+HA-ASK1 sample processed without precipitation.