Figure 1. Transgene constructs and expression analysis in transgenic animal tissues.

(A) Construct design for CD11cTg mice. I-A^bβ was placed under the control of the CD11c promotor with rabbit β-Globin Gene used as a marker for transgene expression (B) Two constructs, one each for I-A^bα and I-A^bβ, were co-injected into MHC-II-deficient (I-E/I-Aβ-deficient). Both MHC-II chains were under control of promotor elements of fatty acid binding protein to direct expression to small intestine (FABPL) (C) Representative dot plots showing staining with anti- MHC-II antibody versus anti-CD11c antibody in splenocytes (top row) and MLN cell suspensions (bottom row) from C57BL/6 (WT), Rag2^−/−, CD11cTg/Rag2^−/− and CD11cNtg/Rag2^−/− mice (indicated in figure). Dot plots are gated through a FSC/SSC gate that excluded small (presumably dead) cells. (D) Histograms show MHC-II expression of CD11c⁺ cells in spleen (left) and MLN (right). (E) RT-PCR showing transgene expression of the I-A^bα construct, the I-A^bβ construct and HPRT in various tissues of EpithTg line 6258.