Table 1.
Flow cytometric analysis of spleens and MLN of CD11cTg/Rag2−/−, CD11cNtg/Rag2−/−, Rag2−/−, and Wt mice.
| Spleen | CD11c+ (%) |
#'s CD11c+ (x 106) |
CD11c+ MHC-II+ (%) |
CD11c+ CD40+ (%) |
CD11c+ CD4+ CD11b− (%) |
CD11c+ CD4− Cd11b− (%) |
CD11c+ CD4− CD11b+ (%) |
CD11c+ CD4+ CD11b+ (%) |
|---|---|---|---|---|---|---|---|---|
|
CD11cTg/ Rag2−/− n=9 |
60.9 ± 5.3** | 6.0 ± 3.0* | 20.0 ± 13.1** | 21.2 ± 8.3* | 2.7 ± 0.5** | 24.8 ± 3.4** | 68.9 ± 3.6** | 1.7 ± 0.8 |
|
Rag2−/− (MHC-II+/+) n=9 |
57.4 ± 7.2** | 6.6 ± 4.9 | 22.3 ± 9.8** | 20.2 ± 9.8** | 1.7 ± 0.7** | 27.3 ± 6.8* | 68.6 ± 6.9** | 0.7 ± 4.1 |
|
CD11cNtg/ Rag2−/− n=4 |
64.4 ± 4.8** | 6.6 ± 3.2* | 0.7 ± 0.9** | 20.3 ± 8.1** | 3.4 ± 1.3** | 21.9 ± 3.6** | 69.9 ± 4.7** | 2.4 ± 1.1 |
|
Wt n=9 |
10.2 ± 6.6 | 11.5 ± 4.3 | 50.6 ± 19.3 | 51.9 ± 10.2 | 11.3 ± 6.7 | 38.8 ± 9.1 | 46.7 ± 15.2 | 1.0 ± 6.7 |
| MLN |
CD11c+ (%) |
#'s CD11c+ (x 105) |
CD11c+ MHC-II+ (%) |
CD11c+ CD40+ (%) |
CD11c+ CD4+ CD11b− (%) |
CD11c+ CD4− Cd11b− (%) |
CD11c+ CD4− CD11b+ (%) |
CD11c+ CD4+ CD11b+ (%) |
|
CD11cTg/ Rag2−/− n=3 |
66.1 ± 9.8** | 4.1 ± 1.8 | 19.4 ± 11.3**£ | 24.1 ± 12.1** | 1.6 ± 0.9** | 35.2 ± 4.3 | 60.8 ± 3.6 | 0.8 ± 0.8** |
|
Rag2−/− (MHC-II+/+) n=7 |
70.9 ± 2.4** | 5.5 ± 1.7 | 66.2 ± 14.7 | 34.7 ± 16.2* | 1.6 ± 0.7 | 42.3 ± 4.7 | 53.6 ± 1.9 | 4.1 ± 3.6 |
|
CD11cNtg/ Rag2−/− n=3 |
63.4 ± 6.8** | 2.4 ± 2.0 | 0.5 ± 0.5** | 31.8 ± 11.9** | 1.4 ± 1.1* | 31.7 ± 5.2 | 64.1 ± 6.9 | 1.1 ± 1.4** |
|
Wt n=5 |
5.9 ± 5.3 | 2.4 ± 2.0 | 68.0 ± 8.4 | 70.4 ± 13.5 | 9.3 ± 6.2 | 46.3 ± 23.4 | 46.5 ± 16.1 | 6.7 ± 1.3 |
*
P<0.05 compared to Wt
**
P<0.01 compared to Wt
£
P<0.01 CD11cTg/Rag2−/− vs Rag2−/− control
MLN-mesenteric lymph nodes