Table 1.
CBDP adducts on residues in human albumin.
| Sequence position |
Peptide sequence | Labeled residue |
Mass of unlabeled peptide, m/z |
Mass of labeled peptide, m/z (mass shift) |
Adduct | Method of identification |
|---|---|---|---|---|---|---|
| 65–73 | SLH*TLFGDK | H67 | 1017.6 | 1123.6 (+106) |
o- Hydroxybenzyl |
LTQ- Orbitrap |
| 65–73 | SLH*TLFGDK | H67 | 1017.6 | 1229.6 (+212) |
Concatenated- [2]-o- hydroxybenzyl |
MALDI MSMS, LTQ- Orbitrap |
| 138–144 | Y*LYEIAR | Y138 | 927.5 | 1097.5 (+170) |
o-Cresyl phosphate |
MALDI MSMS, LTQ- Orbitrap |
| 138–144 | YLY*EIAR | Y140 | 927.5 | 1097.5 (+170) |
o-Cresyl phosphate |
MALDI MSMS, LTQ- Orbitrap |
| 146–159 | H*PYFYAPELLFFAK | H146 | 1742.9 | 1848.9 (+106) |
o- Hydroxybenzyl |
MALDI MSMS, LTQ- Orbitrap |
| 145–159 | RH*PYFYAPELLFFAK | H146 | 1899.0 | 2005.0 (+106) |
o- Hydroxybenzyl |
LTQ- Orbitrap |
| 198–205 | LK*CASLQK | K199 | 947.5 | 1223.6 (+276) |
Ring-opened | MALDI, LTQ- Orbitrap |
| 241–257 | VH*TECCHGDLLECADDR | H242 | 2086.8 | 2192.9 (+106) |
o- Hydroxybenzyl |
MALDI MSMS, LTQ- Orbitrap |
| 246–257 | CH*GDLLECADDR | H247 | 1460.5 | 1566.6 (+106) |
o- Hydroxybenzyl |
LTQ- Orbitrap |
| 338–348 | H*PDYSVVLLLR | H338 | 1311.7 | 1417.8 (+106) |
o- Hydroxybenzyl |
MALDI MSMS, LTQ- Orbitrap |
| 338–348 | H*PDYSVVLLLR | H338 | 1311.7 | 1523.9 (+212) |
Concatenated- [2]-o- hydroxybenzyl |
MALDI, LTQ- Orbitrap |
| 337–348 | RH*PDYSVVLLLR | H338 | 1467.8 | 1573.9 (+106) |
o- Hydroxybenzyl |
MALDI MSMS, LTQ- Orbitrap |
| 349–359 | LAK*TYETTLEK | K351 | 1296.7 | 1572.8 (+276) |
Ring-opened | LTQ- Orbitrap |
| 414–428 | K*VPQVSTPTLVEVSR | K414 | 1640.0 | 1916.0 (+276) |
Ring-opened | LTQ- Orbitrap |
| 429–436 | NLGK*VGSK | K432 | 802.5 | 1078.5 (+276) |
Ring-opened | LTQ- Orbitrap |
| 525–534 | K*QTALVELVK | K525 | 1128.7 | 1234.7 (+106) |
o- Hydroxybenzyl |
LTQ- Orbitrap |
| 525–534 | K*QTALVELVK | K525 | 1128.7 | 1340.8 (+212) |
Concatenated- [2]-o- hydroxybenzyl |
LTQ- Orbitrap |
| 525–534 | K*QTALVELVK | K525 | 1128.7 | 1404.6 (+276) |
Ring-opened | LTQ- Orbitrap |
The asterisk * indicates the adducted residue. Residue numbering is for mature albumin without the signal peptide, as found in accession number gi:122920512 in the NCBI protein database. The complete albumin sequence including Lys 199 is in SwissProt P02768. Monoisotopic masses of singly charged ions are given. Cysteines are carbamidomethylated, adding a mass of +57 amu.