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. Author manuscript; available in PMC: 2012 Aug 26.
Published in final edited form as: Brain Res Bull. 2009 Jan 29;79(2):97–103. doi: 10.1016/j.brainresbull.2009.01.004

Figure 1. MCPIP increases cell death in NT2 cells.

Figure 1

(A) Percent cell death after MCPIP-GFP or GFP (Control) transfection. Cells were harvested at 1, 3, 5 days post transfection. Cell death was detected by trypan blue. (B) Immunoblot analysis of GFP expression and PARP cleavage in NT2 cells transfected with MCPIP-GFP or GFP. Analyzed 5 days post transfection. Protein values were normalized to Beta Actin expression. GFP expression demonstrates equal transfection efficiency. Note: MCPIP-GFP was approximately 90kDa compared to GFP alone (27 kDa). * Indicates statistical significance (**P<0.01, ***P<0.001).