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. Author manuscript; available in PMC: 2012 Aug 26.
Published in final edited form as: Brain Res Bull. 2009 Jan 29;79(2):97–103. doi: 10.1016/j.brainresbull.2009.01.004

Figure 2. Increased glial fibrillary acidic protein expression and morphological changes caused by MCPIP expression.

Figure 2

(A) RT-PCR analysis of GFAP in NT2 cells transfected with MCPIP-GFP or GFP (control) 24 hours post transfection. Figure represents fold change vs. control. (B) Immunoblot analysis of GFAP in cells transfected with MCPIP-GFP or GFP, analyzed 24 hours post transfection. Protein values were normalized to Beta Actin expression. * Indicates statistical significance (*P<0.05, ***P<0.001). Immunocytochemistry showing GFAP expression in NT2 cells transfected with GFP (C) or MCPIP-GFP (D). Analyzed 24 hours post transfection. Scale bar in panel represents 15 μm.