Skip to main content
. Author manuscript; available in PMC: 2012 Aug 26.
Published in final edited form as: Brain Res Bull. 2009 Jan 29;79(2):97–103. doi: 10.1016/j.brainresbull.2009.01.004

Figure 7. APP’s effect on MCP-1 and MCPIP expression relies on p38 MAPK and JAK/STAT signaling.

Figure 7

(A) RT-PCR analysis of MCP-1 and MCPIP in NT2 cells transfected with APP. NT2 cells were pretreated for 45 mins with 50uM of ERK1/2 inhibitor (PD098059), 25uM of p38 MAPK inhibitor (SB203580), 250nM of JAK1 inhibitor, or untreated. NT2 cells were then transfected with APP-GFP or GFP (control). Figure represents fold change over control (GFP). Analyzed 24 hours post transfection. (B) Immunoblot analysis of Phosphorylated ERK (pT202/pY204), p38 MAPK (pT180/pY182), and STAT3 (pY705). Prior to immunoblot analysis cell lysates were immunoprecipitated with anti ERK1/2, p38 MAPK or anti STAT3, respectively. Basal levels of phosphorylation was observed for controls, as expected. * Indicates statistical significance (*P<0.05, **P<0.01, ***P<0.001).