Table IV. [3H]CGS 21680 binding properties of wild type and three epitope-tagged human A2a receptor constructs.
Table shows mean ± S.E. of two or three independent experiments, each performed in duplicate. [3H]CGS 21680 saturation binding studies were carried out with membrane homogenates prepared from transiently transfected COS-7 cells as described under “Experimental Procedures.”
| Constructa | Kd | Bmax |
|---|---|---|
| nm | pmol/mg | |
| A2a (wt) | 15.9 ± 0.3 | 26.9 ± 13.3 |
| Tag1-A2a | 24.1 ± 0.1 | 24.3 ± 12.4 |
| Tag2-A2a | 23.8 ± 2.0 | 28.0 ± 14.4 |
| Tag3-A2a | 21.7 ± 2.6 | 35.4 ± 20.0 |
a
The localization of the HA epitope tag in the various constructs is shown in Fig. 3A.