Table 3.

DSC-Derived Thermodynamic Parameters for the Melting of the G, 8-oxoG, and Gh-Containing Duplexes^a

Duplex	Tmb (°C)	ΔH°calc (kcal/mole)	ΔS°calc (cal/(mole•K))	ΔG°calc,d (kcal/mole)	ΔTm (°C)	ΔΔH°cal (kcal/mole)	ΔΔS°cal (cal/(mole•K))	ΔΔG°cal (kcal/mole)
G	64.3 ± 0.2	103 ± 2	305 ± 6	9 ± 0.5	-	-	-	-
8-oxoG	63.2 ± 0.1	101 ± 2	299 ± 5	8 ± 0.4	−0.9	NDe	NDe	NDe
Gh	47.7 ± 0.1	62 ± 6	193 ± 18	2 ± 0.3	−16.6	−41	−112	−7

^aDNA in 10 mM sodium phosphate, 100 mM NaCl, 0.1 mM EDTA, pH 7.0.

^bT_m reported for a duplex concentration of 32 μM.

^cDerived from DSC thermograms in a model-independent manner as described in the experimental section.

^dValue at 37 °C.

^eNot statistically different from G-containing duplex.