Fig. 2.

p16 protein expression of cultivated human skin fibroblasts of young and elderly persons as well as early and late passage human foreskin fibroblasts.

The protein expression of p16 was assessed in cultivated human skin fibroblasts of young and elderly donors by Western Blot analysis. As controls early and late passage (replicative senescent) human foreskin fibroblasts (HFF) were used. (A) The image shows one representative Western blot analysis of p16 expression of early and late HFF and human skin fibroblasts of young and elderly donors. (B) The graph shows the relative expression of p16 compared to late passage (senescent) HFF. Shown are mean values ± S.E.M; n = 3 (early HFF)/n = 5 (young)/n = 8 (elderly). All values were normalized to expression of the housekeeping gene GAPDH (glyceraldehyde-3-phosphate dehydrogenase).