Fig. 3.

LPS induced IL6 and IL8 secretion of human skin fibroblasts from elderly donors participating in the Leiden 85-plus study.

IL6 (left panel) and IL8 (right panel) concentration in the supernatant of human skin fibroblasts from elderly donors was determined by ELISA after 24 h of stimulation with LPS (10 ng/mL).

White bars: ‘low proinflammatory’ responders, black bars: ‘high proinflammatory’ responders as defined on the basis of the production of proinflammatory cytokines (TNFα, IL1B and IL6) by blood cells following stimulation with LPS for 24 h (van den Biggelaar et al., 2004).