Table 2.
Examples of Real-Time PCR Assays for Detecting Mycoplasma and Ureaplasma Species in Clinical Samples
| Specificity | Target gene/region | Analytical sensitivity (genome copies or CFU/reaction) | Ref. no. |
|---|---|---|---|
| Up and Uu | 16S rRNA | 10 copies | Yoshida et al52 |
| Up | Urease gene subunits and adjacent regions | 5 copies | Mallard et al53 |
| Up | UU063 | 0.6 CFU | Xiao et al22 |
| Up | Urease gene subunits and adjacent regions | 10 copies | Cao et al54 |
| Uu | Urease gene subunits and adjacent regions | 10 copies | Cao et al54 |
| Uu | Urease gene subunits and adjacent regions | 5 copies | Mallard et al53 |
| Uu | UUR10_0680 | 0.8 CFU | Xiao et al22 |
| Mp | P1 | 10 copies | Pitcher et al55 |
| Mp | repMp1 in P1 | 0.2 CFU | Dumke et al32 |
| Mp | CARDS toxin (mpn372) | 1–5 CFU | Winchell et al31 |
| Mp | ATPase operon | 5–50 CFU | Winchell et al31 |
| Mp | 16S rRNA | 1 copy | Khanna et al45 |
| Mp | 16S rRNA | 5 CFU | Raggam et al44 |
| Mg | gap | 5 copies | Svenstrup et al56 |
| Mg | 16S rRNA | 10 copies | Yoshida et al57 |
| Mh | gap | 10 copies | Baczynska et al58 |
| Mh | yidC | 7 copies | Ferandon et al59 |
Mg, M. genitalium; Mh, M. hominis; Mp, M. pneumoniae; Up, U. parvum; Uu, U. urealyticum.