Figure 1.

Saccular hair cells were eliminated by noise exposure and subsequently regenerated. A, Hair cell loss in the saccule was consistently induced by the noise exposure apparatus. B, Phalloidin staining of the saccular hair cells; hair cell loss occurred in the anterior-medial area of the saccular sensory epithelium (red boxes; scale bar, 100 μm). C, Closer examination of the damaged area with phalloidin (green channel) and anti-myosin VI/VIIa (red channel) staining confirmed the complete elimination of hair cell bodies (middle; scale bar, 20 μm) as well as the repopulation of hair cells to control level by 96 hpe in the damaged area (bottom). D, Quantification of hair cell number after sound exposure (one-way ANOVA and post hoc test, n = 3, *p < 0.05; all error bars in this publication demonstrate SD). D, Dorsal; A, anterior.