Figure 4.

Hair cell death in the lateral line system resulted in an increase in stat3 expression level in the nonsensory cells in the neuromasts. A, The results of regular whole-mount in situ hybridization targeting stat3 mRNA in control (top) CuSO₄-treated (bottom) larvae showed an increase in stat3 expression when hair cell death took place. Scale bar, 1 mm. B, Costaining of neuromasts with fluorescent detection of probes targeting stat3 mRNA (red channel) and FITC-conjugated anti-GFP antibody (green channel) in ET20 transgenic larvae showed an increase in nonsensory cells in the neuromasts after hair cell death. Scale bar, 20 μm.