Table 3.
GluC V8 glycopeptide digests identified during LC-ESI-QqTOF-MS/MS analysis of the hapten-BSA glycoconjugate with a hapten:BSA ratio of 4.3:1, 6.6:1 and 13.2:1
| Peptide | Missed | Hapten:BSA ratio 4.3:1 | Hapten:BSA ratio 6.6:1 | Hapten:BSA ratio 13.2:1 | ||||
|---|---|---|---|---|---|---|---|---|
| Sequence (star = glycation site) | Calculated | Observed | Deviation | Observed | Deviation | Observed | Deviation | |
| m/z (charge) | cleavage | m/z (charge) | (Da) | m/z (charge) | (Da) | m/z (charge) | (Da) | |
| K*SHCIAE (Lys 309) | 679.3189 (+2) | 0 | 679.3320 (+2) | 0.0131 | 679.3343 (+2) | 0.0154 | 679.3284 (+2) | 0.0095 |
| LTKVHK*E (Lys 266) | 684.3745 (+2) | 0 | 684.3867 (+2) | 0.0122 | 684.3851 (+2) | 0.0106 | 684.3795 (+2) | 0.0050 |
| K*VTKCCTE (Lys 495) | 769.8575 (+2) | 0 | 769.8677 (+2) | 0.0102 | 769.8720 (+2) | 0.0145 | 769.8668 (+2) | 0.0093 |
| K*QEPERNE (Lys 117) | 771.8678 (+2) | 2 | 771.8852 (+2) | 0.0174 | 771.8795 (+2) | 0.0117 | 771.8883 (+2) | 0.0205 |
| K*SLHTLFGDE (Lys 88) | 830.4093 (+2) | 1 | 830.4932 (+2) | 0.0839 | 830.4246 (+2) | 0.0153 | 830.4196 (+2) | 0.0103 |
| DKGACLLPK*IE (Lys 204) | 878.9556 (+2) | 0 | 878.9783 (+2) | 0.0227 | 878.9669 (+2) | 0.0113 | 878.9643 (+2) | 0.0087 |
| AK*DAFLGSFLYE (Lys 346) | 937.4590 (+2) | 1 | 937.4871 (+2) | 0.0281 | 937.4759 (+2) | 0.0169 | 937.4798 (+2) | 0.0208 |
| KK*FWGKYLYE (Lys 156) | 937.9824 (+2) | 0 | 937.9877 (+2) | 0.0053 | 937.9862 (+2) | 0.0038 | 937.9648 (+2) | −0.0176 |
| LLYYANK*YNGVFQE (Lys 183) | 1118.0465 (+2) | 0 | 1118.0711 (+2) | 0.0246 | 1118.0587 (+2) | 0.0122 | 1118.0511 (+2) | 0.0046 |
| VSRSLGK*VGTRCCTKPE (Lys 455) | 816.7456 (+3) | 0 | 816.7646 (+3) | 0.0190 | 816.7583 (+3) | 0.0127 | 816.7483 (+3) | 0.0027 |
| K*TPVSE (Lys 489) | 587.2979 (+2) | 0 | - | - | 587.3103 (+2) | 0.0124 | 587.3041 (+2) | 0.0062 |
| GPK*LVVSTQTALA (Lys 597) | 899.4959 (+2) | 0 | - | - | 899.5080 (+2) | 0.0121 | 899.5092 (+2) | 0.0133 |
| LLKHKPK*ATEE (Lys 561) | 903.9960 (+2) | 1 | - | - | 904.0190 (+2) | 0.0230 | 903.9916 (+2) | −0.0044 |
| K*QIKKQTALVE (Lys 544) | 900.0117 (+2) | 0 | - | - | 900.0234 (+2) | 0.0117 | 900.0165 (+2) | 0.0048 |
| VTK*LVTD (Lys 256) | 644.8478 (+2) | 0 | - | - | - | - | 644.8498 (+2) | 0.0020 |
| HVK*LVNE (Lys 65) | 676.3588 (+2) | 0 | - | - | - | - | 676.3647 (+2) | 0.0059 |
| VEK*DAIPE (Lys 318) | 707.3534 (+2) | 2 | - | - | - | - | 707.3578 (+2) | 0.0044 |
| CCDK*PLLE (Lys 304) | 774.3520 (+2) | 1 | - | - | - | - | 774.3662 (+2) | 0.0142 |
| LCK*VASLRE (Lys 100) | 794.9162 (+2) | 0 | - | - | - | - | 794.9163 (+2) | 0.0001 |
| VSRSLGK*VGTRCCTK*PE (Lys 455, Lys 463) | 987.8230 (+3) | 0 | - | - | - | - | 987.8313 (+3) | 0.0083 |
| KK*FWGK*YLYE (Lys 156, 160) | 1194.5985 (+2) | 0 | - | - | - | - | 1194.5626 (+2) | −0.0359 |
| NFVAFVDK*CCAADDKE (Lys 580) | 1201.5300 (+2) | 3 | - | - | - | - | 1201.5350 (+2) | 0.0050 |