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NO production in transiently silenced ZmCCaMK protoplasts. The protoplasts were treated with 10 µM ABA for 30min, and then loaded with 4,5-diaminofluorescein diacetate (DAF-2DA) and detected by confocal laser scanning microscopy. Values are the mean ±SE of three different experiments. Means denoted by the same letter did not differ significantly at P < 0.05 according to Duncan’s multiple range test.
