Fig. 2.

(A) Omega-3 fatty acid desaturase gene expression in mature leaves from soybean plants kept at control temperature (24 °C); 5 °C exposure for 24, 48, and 72h; and after recovery for 4 days at control temperature. GmSCOF-1 was used as an internal control for cold-inducible expression. ACTIN was used as a housekeeping gene in all experiments. (B) Normalization of gene expression results against ACTIN. Data are mean ± SD from two experiments. For the same time point, different letters indicate significant differences among treatments (P < 0.05).