Fig. 9.

Isoflurane (Iso) decreases glutamate concentration in the media during profound hypothermia (PH) and rewarming (RW). Glutamate was assayed in control cultures and after 6 h at 4°C followed by 1h of rewarming (PH/RW), and with 2% isoflurane in the gas phase during the entire PH/RW period. The KCl group was treated with 80 mM KCl at 37°C to cause maximal glutamate release. In all panels, the number of cultures in each treatment group are indicated with numbers above the bars. The lines and asterisks above the error bars describe statistical differences between different treatment groups (p<0.05), measured with ANOVA and a Tukey multiple comparison post test.