TABLE 3.
Sensitivity of detection of purified DNA from a pure culture of V. vulnificus MO6-24(O) by using real-time PCR and SYBR Green I fluorescent dyea
| Sampleb | Amt of DNA | Ct value | Melting temp (°C) |
|---|---|---|---|
| 2 (positive control) | 15.19 ± 1.8 | 87.0 ± 0.04 | |
| 3 | 0.1 μg | 17.33 ± 1.2 | 87.68 ± 0.03 |
| 4 | 0.01 μg | 20.65 ± 1.5 | 87.07 ± 0.3 |
| 5 | 1 ng | 24.8 ± 1.4 | 87.93 ± 0.02 |
| 6 | 0.1 ng | 27.53 ± 1.02 | 86.04 ± 1.03 |
| 7 | 0.01 ng | 32.09 ± 1.2 | 87.5 ± 0.06 |
| 8 | 1 pg | 38.95 ± 0.38 | 86.92 ± 0.02 |
| 9 | 0.1 pg | 77.01 ± 1.05 |
a
The data are means ± standard deviations for 10 independent experiments.
b
The sample numbers correspond to the lane numbers in Fig. 1C.