TABLE 6.
Detection of V. vulnificus MO6-24(O) from unseeded gulf water following 5 h of enrichmenta
| Sampleb | Ct value | Melting temp (°C) | Calculated concn (CFU/ml) | Viable plate count (CFU/ml) |
|---|---|---|---|---|
| 1 | 20.9 ± 1.5 | 87.4 ± 0.05 | 3 | 4 ± 1 |
| 2 | 18.48 ± 0.85 | 87.6 ± 0.12 | 29 | 32 ± 3.5 |
| 3 | 17.53 ± 1.34 | 87.4 ± 0.2 | 76 | 81 ± 4.72 |
| 4 | 17.95 ± 2.2 | 86.9 ± 0.4 | 52 | 58 ± 4.5 |
| 5 | 20.39 ± 1.66 | 87.2 ± 0.05 | 4 | 3 ± 2 |
| Positive control | 12.37 ± 0.53 | 87.1 ± 0.08 | ||
| Negative control | 27.34 ± 0.6 | 76.89 ± 0.05 |
a
The values are means ± standard deviations for three independent experiments.
b
The samples were collected in the Gulf of Mexico off the coast of Dauphin Island in Alabama.