Fig. 5.

EBOV GP-specific CD8+ T cell responses detected in peripheral blood samples following vaccination. PBMCs from various time points were stimulated with a peptide pool spanning the entire EBOV GP protein in the presence of GolgiPlug (brefeldin A), and then stained for CD3, CD4, and CD8 surface T cell markers. Cells were then fixed, permeabilized, and stained for intracellular accumulation of (A) IFNγ or (B) TNFα as indicators of their activation. We did not detect accumulation of either cytokine in CD4+ T cells (not shown).