Table 1.
Genes regulated by Bcl-2 tumoral environment on the HMVEC endothelial cell line (P < 0.001)
| Genes | Affymetrix ID | Cellular localization | Function(s) | Org 6 media vs. PC-3 media | Org 6 media vs. DMEM |
|---|---|---|---|---|---|
| Direction of regulation (fold change) | Direction of regulation (fold change) | ||||
| Caspase 1 | 211366_x_at | Cytoplasma | Proteolysis, apoptosis | ⇑ (41) | ⇑ (3) |
| Janus kinase 3 | 227677_at | Cell membrane | Amino acid phosphorylation, male gonad development | ⇑ (4) | ⇑ (3) |
| Superoxide dismutase 2, mitochondrial | 216841_s_at | Mitochondrion | Regulation of transcription, response to oxidative stress | ⇑ (9) | ⇑ (2) |
| Stanniocalcin 2 | 203438_at | Secreted | Receptor linked signal transduction, cell–cell signaling | ⇑ (6) | ⇑ (7) |
| Arresting domain containing 4 | 225283_at | – | – | ⇑ (5) | ⇑ (4) |
| Thrombospondin, type I, domain containing 4 | 226506_at | Secreted | Metalloendopeptidase activity | ⇑ (4) | ⇑ (2) |
| Proliferation-inducing protein 38 | 219501_at | Cell membrane, secreted | – | ⇑ (4) | ⇑ (2) |
| Muskelin 1 | 204423_at | Cytoplasma | Cell motility, cell–matrix adhesion | ⇓ (0.5) | ⇓ (0.5) |
| Cingulin-like 1 | 225817_at | – | – | ⇓ (0.3) | ⇓ (0.4) |
| Periostin, osteoblast specific factor | 210809_s_at | Secreted | Skeletal development, cell adhesion | ⇓ (0.3) | ⇓ (0.5) |
| Carbonic anhydrase II | 209301_at | Cytoplasma | Carbon dioxide transport, secretion | ⇓ (0.3) | ⇓ (0.3) |
| Growth differentiation factor 3 | 220053_at | Secreted | Cytokine activity, growth factor activity | ⇓ (0.3) | ⇓ (0.5) |
| P8 protein (candidate of metastasis 1) | 209230_s_at | Nucleus | Apoptosis, cell growth | ⇑ (2) | ⇑ (3) |
| Intercellular adhesion molecule 1 | 20263 8_s_at | Cell membrane | Cell–matrix adhesion, receptor linked signal transduction, cell–cell signaling | ⇑ (6) | ⇑ (3) |
| Growth differentiation factor 15 | 221577_x_at | Secreted | Regulation of transcription, TGFbeta signaling, chromatin modification | ⇑ (3) | ⇑ (3) |
| Interleukin 8 | 202859_x_at | Secreted | Angiogenesis, cell motility, chemotaxis | ⇑ (7) | ⇑ (7) |
| Chemokine ligand 2 | 209774_x_at | Secreted | Chemotaxis, inflammatory response | ⇑ (22) | ⇑ (2) |
| Growth arrest-specific 1 | 204457_s_at | Cell membrane | Apoptosis, cell cycle | ⇓ (0.4) | ⇓ (0.4) |
| Desmuslin | 212730_at | Cytoplasma | – | ⇓ (0.2) | ⇓ (0.3) |
| p21 (CDKN1A)-activated kinase 2 | 208875_s_at | Nucleus, cytoplasm | Translation, signal transduction | ⇓ (0.4) | ⇓ (0.5) |
| Oxytocin receptor | 206825_at | Cell membrane | Muscle contraction, signal transduction | ⇓ (0.1) | ⇓ (0.2) |
Functional groups of genes were assigned using gene-association files from the GO Consortium and by GenMapp and Kegg pathway analysis. Full gene list is available at http://genomics.biotech.ufl.edu/people/rosser/