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. 2012 Aug 1;32(31):10587–10593. doi: 10.1523/JNEUROSCI.0015-12.2012

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Copyright © 2012 the authors 0270-6474/12/3210587-07$15.00/0

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Figure 2. — FRAP of RHO and IFT88 in cilia of hTERT-RPE1 cells. A, Cells cotransfected to express either RHO-EGFP with ARL13B-mCherry or IFT88-YFP with ARL13B-mCherry. ARL13B-mCherry was used as a live-cell cilium marker. Right, Shows overlays. Scale bar, 5 μm. B, Images illustrating fluorescence recovery of RHO-EGFP and IFT88-YFP in the distal cilium. Pre, Prebleach; Post, recovery 3 min after photobleaching. Scale bar, 1 μm. C, D, Examples of plots of normalized fluorescence recovery in the distal cilium vs time.