Figure 2. FAM83B drives AIG in HME1 cells and is elevated in human cancer specimens.

(A and B) HME1 cells were infected with a retrovirus encoding full-length FAM83B, VBIM-FAM83B, or a control retrovirus and analyzed for AIG. Original magnification, ×10. (C) Western analysis for FAM83B expression. (D) Microarray data sets E-MEXP-882 (4 normal, 18 cancerous) and E-TABM-276 (4 normal, 13 cancerous), acquired from Array Express, were used to examine transcriptional changes between normal (N) and cancerous (C) human breast tissues. (E and F) Origene Tissue­Scan Cancer Panels (48-well breast cancer panels and a 384-well multi-cancer survey panel) were analyzed by real-time PCR for FAM83B. Shown is relative FAM83B expression for specimens that had a statistically significant difference between cancerous and associated normal tissues. n = 2 (normal bladder), 3 (normal thyroid and normal ovary), 4 (normal lung and normal cervix), 6 (normal testis), 9 (cancerous cervix), 16 (normal breast), 18 (cancerous thyroid), 19 (cancerous lung and cancerous testis), 21 (cancerous ovary), 22 (cancerous bladder), 191 (cancerous breast). (G) Relative expression of FAM83B in a panel of matched lung cancer and associated normal lung tissues. Each normal tissue was set equal to 1.