Figure 11. Ly6G⁺ cell depletion suppresses AOM/DSS-induced and spontaneous intestinal tumorigenesis.

(A–C) WT Balb/c mice were injected with AOM and then underwent 2 5-day periods of 2% DSS feeding, interspersed by 16 days on normal water. From the start of the first DSS feed, groups of 10 mice were treated 3 times per week with either 1A8 (anti-Ly6G) or isotype control (2A3) Ab. (A) Number of colonic polyps per mouse, polyp size, and overall tumor burden per mouse. (B) Blood neutrophil counts, expressed as total and percentage, on the day of sacrifice (n = 5 [isotype]; 4 [1A8]). (C) Microvessel density in adenomas (n = 3 per group). (D–G) Apc^Min/+ mice were treated 3 times per week with either 1A8 (anti-Ly6G) or isotype control (2A3) Ab from 5 weeks of age, and sacrificed 50 days later. (D) Number of polyps per mouse. (E) Representative sections of adenomas immunostained with Ab against MPO (brown), counterstained with hematoxylin, and visualized by light microscopy. Arrows denote clusters of MPO⁺ cells. Original magnification, ×200. (F) Number of MPO⁺ neutrophils per polyp section. (G) Microvessel density in adenomas (n = 5–6 per group). *P < 0.05, **P < 0.01, ***P < 0.001, Mann-Whitney test. Box and whisker plots show median (lines within boxes), interquartile range (bounds of boxes), and upper and lower range (whiskers).