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. 2012 Aug 27;122(9):3127–3144. doi: 10.1172/JCI61067

Figure 5. Cxcr2 deficiency protects against intestinal inflammation induced by 2% DSS.

Figure 5

WT and Cxcr2–/– mice were fed 2% DSS for 5 days and then normal water for a further 6 days. (A) Relative expression of Cxcr2 and its ligands in WT Balb/c colon during 2% DSS feeding, as determined by Q-RT-PCR; mean expression in colon of untreated control mice (UC) is set to 1. On refers to duration of 2% DSS feeding; Off refers to duration of return to normal water. *P < 0.05, **P < 0.01 versus untreated control, 1-way ANOVA with multiple comparison post-test. (B and C) Representative sections of colons from WT and Cxcr2–/– Balb/c mice immunostained with anti-MPO (B) or anti-BrdU (C) Ab (brown), either 2 days after returning to normal water or in the absence of DSS exposure. Mice were injected i.p. with BrdU 2 hours before harvest. Sections were counterstained with hematoxylin and visualized by light microscopy. The number of MPO+ cells per section of WT and Cxcr2–/– (KO) colons after 5 days of 2% DSS feeding and 2 days after return to normal water (n = 4 per group) is also shown in B. *P < 0.05, **P < 0.01, Mann-Whitney test. Scale bars: 200 μm (B and C). Box and whisker plots show median (lines within boxes), interquartile range (bounds of boxes), and upper and lower range (whiskers).