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. 2012 Aug 6;122(9):3248–3259. doi: 10.1172/JCI61216

Figure 6. Inhibition of Notch signaling increases expression of WNT target genes in CRCLs.

Figure 6

(A) Real-time qRT-PCR analysis of WNT target genes PROX1 in Nrarphi CRCLs. SNU1040, SNU61, SNU283, LOVO, and SW480 cells were treated daily with vehicle or 1 εM DAPT (upper graphs). Each cell was transfected with 2 εg of mock vector or DN-MAML (dominant-negative form of MAML) (lower graphs). Levels of PROX1 transcripts were measured at the indicated times (72 and 96 hours). Bars indicate mean + SD. (B) Real-time qRT-PCR analysis of WNT target genes in DAPT-treated CRCLs. LOVO (upper graphs) and SNU61 (lower graphs) cells were treated daily with vehicle or 1 εM DATP. Levels of Axin2 and c-MYC transcripts were measured at the indicated times (72 and 96 hours). MMP2 and Nrarp were used for the control and Notch target genes, respectively. β-Actin was used for normalization. Bars indicate mean + SD. *P < 0.05; **P < 0.001.