Fig. 1.

A schematic diagram for a proteomics workflow. A typical workflow for a high-throughput identification and quantification proteomics experiment. The workflow consists of four major steps: sample collection, protein extraction and purification, sample fractionation (in either protein or peptide space) and mass spectrometry analysis. Various entry points for quantitative proteomics are shown by arrowed boxes. The collected raw mass spectra data are then analysed by various bioinformatics pipelines illustrated in Fig. 2.