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Relative mRNA expression of Nrf2 and its regulators Keap1 and DJ-1. Real-time PCR analysis of mRNA extracted from normal donor corneal endothelium and from age- and sex-matched FECD specimens obtained at time of keratoplasty. Results were expressed as fold-changes and were normalized to β2-microglobulin. Data showed transcriptional downregulation of DJ-1 in FECD, whereas Nrf2 and Keap1 mRNA were unaffected. Data are represented as mean ± SEM. *P < 0.05.
