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. Author manuscript; available in PMC: 2012 Aug 27.

Published in final edited form as: Clin Cancer Res. 2008 Jul 1;14(13):4284–4291. doi: 10.1158/1078-0432.CCR-07-5226

(A) 1483, (B) PCI-37B, and (C) Cal-33 cells were plated in 12-well plates at a density of 3 × 10⁴ cells/well in DMEM with 10% FBS. After 24 hours, growth media was replaced with media containing DMSO, AZD0530 (at IC₅₀ value), gefitinib (at IC₅₀ value), or a combination of AZD0530 and gefitinib. On days 0, 2, 4, 6 and 8, viable cells were counted using vital dye exclusion. Four independent experiments (in triplicate) were performed for each cell line. Cell numbers on day 8 after treatment are shown.

(A) 1483, (B) PCI-37B, and (C) Cal-33 cells were plated in 12-well plates at a density of 3 × 10⁴ cells/well in DMEM with 10% FBS. After 24 hours, growth media was replaced with media containing DMSO, AZD0530 (at IC₅₀ value), gefitinib (at IC₅₀ value), or a combination of AZD0530 and gefitinib. On days 0, 2, 4, 6 and 8, viable cells were counted using vital dye exclusion. Four independent experiments (in triplicate) were performed for each cell line. Cell numbers on day 8 after treatment are shown.