Table 2.
Chemical composition of excreted FFA from GC/MS analysis.
| % of total peak area | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Strain | 14:0 | 16:0 | 16:1 | Unknown 1 | 18:0 | 18:1 (Δ9 cis) | 18:1 (Δ11 cis) | Unknown 2 | Total (%) |
| 7942 | 1.66 ± 0.93 | 42.8 ± 6.07 | 16.1 ± 4.46 | ND | 19.1 ± 6.60 | 6.78 ± 2.91 | 13.6 ± 6.86 | ND | 100 |
| SE01 | 0.82 ± 0.23 | 22.8 ± 7.38 | 19.8 ± 9.38 | 11.7 ± 3.59 | 11.4 ± 3.64 | 7.57 ± 1.25 | 17.9 ± 2.24 | 8.01 ± 2.72 | 100 |
| SE02 | 0.94 ± 0.11 | 26.3 ± 4.04 | 14.0 ± 4.02 | 11.2 ± 8.02 | 15.7 ± 6.27 | 5.99 ± 1.24 | 21.1 ± 2.62 | 4.79 ± 0.28 | 100 |
Percentages are based on total peak area from GC analysis; MS spectra were used to confirm peak identities. Data are reported as averages of 3 biological replicates with standard deviation. While the amount of FFA excreted by the wild type is negligible due to recycling via acyl-ACP synthetase, FFA are detected from GC/MS analysis, most likely due to contaminating membrane components found in the supernatant.