Table 1.
List of proteins that were secreted by B16F0 cell line after 24 hours in serum free media, identified using 2D-GE/MALDI-TOF MS workflow, and formed the data set for IPA analysis. Proteins that were used as focus proteins in the protein interaction network (bolded) and that are known to be associated with exosomes (‡) are highlighted. The identification summary of these proteins including the peptide hits and sequence coverage are listed in Supplemental File 2.
| SPOT ID |
Protein Name | GENE SYMBOL |
Theoretical | |
|---|---|---|---|---|
| MW | pI | |||
| 1 | Endoplasmin | Hsp90b1‡ | 90 | 4.7 |
| 2 | Heat shock protein HSP 90-alpha | Hsp90aa1‡ | 85 | 4.9 |
| 4 | Calreticulin | Calr‡ | 46 | 4.3 |
| 5 | Rab GTPase-binding effector protein 2 | Rabep2 | 62 | 4.9 |
| 6 | Protein disulfide-isomerase | P4hb‡ | 55 | 4.8 |
| 8,35 | ATP synthase subunit beta, mitochondrial | Atp5b | 52 | 5 |
| 9 | SPARC | Sparc | 33 | 4.7 |
| 10 | Annexin A5 | Anxa5‡ | 36 | 4.8 |
| 14 | ATP synthase subunit d, mitochondrial | Atp5h | 19 | 5.5 |
| 15 | 26S proteasome non-ATPase regulatory subunit 10 | Psmd10 | 25 | 5.7 |
| 16 | N-acetyltransferase 9 | Nat9 | 28 | 5.2 |
| 17 | Prohibitin | Phb‡ | 30 | 5.5 |
| 18 | TIP41-like protein | Tiprl | 31 | 5.4 |
| 19 | Nuclear distribution protein nudE homolog 1 | Nde1 | 36 | 5.3 |
| 20 | Annexin A4 | Anxa4‡ | 36 | 5.4 |
| 25,28,29 | T-complex protein 1 subunit theta | Cct8‡ | 59 | 5.4 |
| 30 | RuvB-like 2 | Ruvbl2‡ | 51 | 5.5 |
| 32 | Tubulin alpha-1C chain | Tuba1c‡ | 50 | 5 |
| 33 | Na(+)/H(+) exchange regulatory cofactor NHE-RF3 | Pdzk1‡ | 56 | 5.3 |
| 36,37,38 | Actin, cytoplasmic 2 | Actg1‡ | 42 | 5.3 |
| 40 | Homer protein homolog 1 | Homer1 | 41 | 5.4 |
| 41 | Activator of 90 kDa heat shock protein ATPase homolog 1 | Ahsa1‡ | 38 | 5.4 |
| 42 | Guanine nucleotide-binding protein subunit alpha-11 | Gna11‡ | 42 | 5.7 |
| 43,45 | Protein disulfide-isomerase A3 | Pdia3‡ | 54 | 5.7 |
| 44 | Baculoviral IAP repeat-containing protein 4 | Xiap | 56 | 5.7 |
| 47 | Annexin A6 | Anxa6‡ | 76 | 5.3 |
| 48 | RNA polymerase I-specific transcription initiation factor | Rrn3 | 75 | 5.3 |
| 49 | Collagen type IV alpha-3-binding protein | Col4a3bp | 71 | 5.3 |
| 50 | Calpain-9 | Capn9 | 79 | 5.1 |
| 51 | Heat shock cognate 71 kDa protein | Hspa8‡ | 71 | 5.4 |
| 52 | Cytochrome P450 2D11 | Cyp2d11 | 57 | 6.2 |
| 53 | Pyruvate kinase isozymes M1/M2 | Pkm2‡ | 58 | 6.7 |
| 54 | 5-azacytidine-induced protein 2 | Azi2 | 46 | 6.2 |
| 56 | Ornithine aminotransferase, mitochondrial | Oat | 46 | 5.7 |
| 57 | Transcobalamin-2 | Tcn2‡ | 46 | 5.9 |
| 58 | WNT1-inducible-signaling pathway protein 1 | Wisp1 | 43 | 6 |
| 60 | Alpha-enolase | Eno1‡ | 47 | 6.4 |
| 62 | Cytosolic 5’-nucleotidase 3 | Nt5c3 | 37 | 6.2 |
| 63 | Ankyrin repeat domain-containing protein 16 | Ankrd16 | 40 | 6.5 |