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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: J Immunol. 2012 Jul 9;189(4):1737–1746. doi: 10.4049/jimmunol.1103541

Figure 3. TOLLIP SNP rs5743899 and cytokine responses after TLR and MTb stimulation of peripheral blood mononuclear cells.

Figure 3

Peripheral blood mononuclear cells were isolated from 64 healthy volunteers in Seattle and stimulated with media or TLR ligands (LPS at 10 ng/ml, PAM2 at 250 ng/ml, PAM3 at 250 ng/ml, MTb whole cell lysate at 1 µg/ml) for 24 hours. Secreted A) IL-6 and B) IL-10 levels were measured in supernatants via ELISA. C–F) IL-6 responses after TLR stimulation, stratified by rs5743899 genotype. AA individuals = 35, AG = 21, GG = 7. G–J) IL-10 responses after TLR stimulation, stratified by rs5743899 genotype. * p<0.01; ** p=0.03 by Mann-Whitney test in a recessive model.