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Image analysis of hBMSC morphology during culture on un-etched and etched scaffolds (1 d). Confocal fluorescence images of actin-stained hBMSCs (Alexa Fluor 546 phalloidin) were analyzed to quantify cell area (a), perimeter (b), aspect ratio (c), roundness (d) and circularity (e). Maximum intensity Z-projections were used for analysis. Error bars are S.D. (n = 10 cells). Asterisks indicate significant differences (p < 0.05, t-test).
