Table 2.
Main features of immunoglobulins characterized from representative teleost fish
| Body fluid | Species | References | Ig isotype | Native form (based on GF) |
GF/SDS-PAGE size (kDa) |
H/L chain size (kDa) |
Concentration (mg ml−1) |
|---|---|---|---|---|---|---|---|
| Serum | Rainbow trout (O. mykiss) |
(Elcombe et al., 1985),(Zhang et al., 2010) | HMW/IgM LMW/IgT |
Tetramer Monomer |
~700/700 ~180/180 |
~75/25 ~70/25 |
2.52 0.004 |
| Common carp (C. carpio) |
(Rombout et al., 1986),(Rombout et al., 1993a) | HMW | Tetramer | ~/760,380,190 | ~70/25 | 1.73 | |
| Sheepshead (A. probatocephalus) |
(Lobb and Clem, 1981d),(Lobb and Clem, 1981a) | HMW LMW |
Tetramer Monomer |
~700/700,350 ~140/70 |
~70/25 ~45/25 |
2.90 0.17 |
|
| Gut mucus or Bile |
Rainbow trout (O. mykiss) |
(Zhang et al., 2010) | IgM IgT |
Tetramer Tetramer |
~700/700 ~700/180 |
~75/25 ~70/25 |
0.075 0.007 |
| Sheepshead (A. probatocephalus) |
(Lobb and Clem, 1981b),(Lobb and Clem, 1981a) | Bile Ig | Dimer | ~320/160 | ~55/25 | 0.09 | |
| Skin mucus | Rainbow trout (O. mykiss) |
(Bromage et al., 2006) | IgM | Tetramer | ~800/800,600,400, 200,100 |
~72/25 | N/A |
| Common carp (C. carpio) |
(Rombout et al., 1986),(Rombout et al., 1993a) | HMW | Tetramer | ~/760,380,190 | ~70/25 | 0.008 | |
| Sheepshead (A. probatocephalus) |
(Lobb and Clem, 1981c),(Lobb and Clem, 1981a) | HMW Dimeric Ig |
Tetramer Dimer |
~700/700 ~400/400, 350/175 |
~70/25 ~70/25 |
0.09 0.024 |
Abbreviations: HMW = high molecular weight Ig, LMW = low molecular weight Ig, C = covalent, NC = noncovalent, GF = gel filtration, Ab = antibody, mAb = monoclonal antibody, pAb = polyclonal antibody.